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Tethered particle motion (TPM) is a biophysical method that is used for studying various polymers such as DNA and their interaction with other entities such as proteins. The method allows observers to measure various physical properties on the substances, as well as to measure the properties of biochemical interactions with other substances such as proteins and enzymes. TPM is a single molecule experiment method. ==History== TPM was first introduced by Schafer, Gelles, Sheetz and Landick in 1991.〔(Schafer, D.A., et al., ''Transcription by single molecules of RNA polymerase observed by light microscopy.'' Nature, 1991. 352: p. 444-448. )〕 In their research, they attached RNA polymerase to the surface, and gold beads were attached to one end of the DNA molecules. In the beginning, the RNA polymerase "captures" the DNA near the gold bead. During the transcription, the DNA "slides" on the RNA polymerase so the distance between the RNA polymerase and the gold bead (the tether length)is increased. Using an optical microscope the area that the bead moves in was detected. The transcription rate was extracted from data. Since then, a lot of TPM experiments have been done, and the method was improved in many ways such as beads types, biochemstry techniques, imaging (faster cameras, different microscopy methods etc.) data analysis and combination with other single-molecule techniques (e.g. optical or magnetical tweezers). 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Tethered particle motion」の詳細全文を読む スポンサード リンク
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